Bosco Ngusi, a resident of Hakabungo cell in Mukaranje ward, Katuna town council is said to have committed the offence on December 12, this year.
The Local Council Three chairperson for Katuna town council, Nelson Nshangabashaija, said Ngusi used a syringe to get infected blood from his body before him injecting it to the victims who are both four years of age.
Ngusi is believed to have acquired the virus from his late parents.
According to the police spokesperson for Kigezi region Elly Maate who confirmed the arrest, Ngusi was arrested on Saturday from his hideout in Katuna town council.
He is currently detained at Kabale central Police station.